CHANGES IN HAEMATOLOGICAL PARAMETERS IN STREPTOZOTOCIN-INDUCED DIABETIC ALBINO RATS TREATED WITH AQUEOUS EXTRACT OF HYPOESTES ROSEA LEAF
Africa P. E.*, Nwachuku E. O., Tamuno-Emine D. G. and Bartimaeus E. S.
ABSTRACT
The aim of this study was to assess the effect of Hypoestes rosea on some haematological parameters packed cell volume; haemoglobin; red blood cell; white blood cell; neutrophil; lymphocyte, monocyte; eosinophil; platelet; mean corpuscular haemoglobin concentration; mean corpuscular haemoglobin; mean corpuscular volume in streptozotocin induced diabetic rats. A total of hundred and sixteen (116) albino rats were assigned by weight into eighteen (18) groups. The duration of the study was fifteen (15) days for acute and thirty (30) days sub-chronic. The study groups comprised of two (2) treatment phases each (prophylactic and therapeutic) with nine (9) experimental groups in each of the study group. Ten (10) rats each were assigned for the two (2) positive control groups and six (6) rats each were assigned to the other experimental groups. These groups with an average body weight of 201±.65.20 to 232 ± 16.23 g were composed as follows: Healthy rats receiving de-ionized water (negative control); diabetic rats administered with streptozotocin (Positive Control); healthy rats receiving aqueous extract orally only (EC 100 mg/kg body weight) and diabetic rats administered with aqueous extract orally daily for fifteen (15) days and thirty (30) days (AEHR 100 ng/kg body weight, 200 mg/kg body weight and 300 mg/kg body weight; (diabetic treated group). Animals were fasted for 16 hr, weighed and painlessly sacrificed through the jugular vein on day sixteen (16) and thirty-one (31) respectively after the experimental phases. Blood samples were collected for the determination of haematological parameters using Automated Analyzer, Sysmex (XS-1000i) which principle is based on flow cytometry. Results showed that there were no significant difference between haematological parameters of streptozotocin induced rat group and those of the different dose-treated groups. This indicates that treatment with aqueous extract of hypoestes rosea does not affect haematological parameters in streptozotocin induced diabetes rats.
Keywords: Haematological, Hypoestes rosea, Streptozotocin-Induced, Diabetic Albino Rats.
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