European Journal of Biomedical and Pharmaceutical Sciences

A COMPARATIVE STUDY ON VALIDATION OF RANOLAZINE AND LACOSAMIDE IN PHARMACEUTICAL FORMULATIONS BY RP-HPLC METHOD

Masma Shaik* and Y. Sunandamma

ABSTRACT

A reversed phase high performance liquid chromatographic (RP-HPLC) method was developed for the determination of ranolazine and lacosamide in pharmaceutical formulations. A comparative study was made on the results obtained to evaluate the strong and weak aspects of the reported method. Hypersil BDS-C8 column with sodium acetate-acetonitrile (50:50) mobile phase was employed for the determination of ranolazine in the range 0 to 150 μg mL-1. Completely resolved peaks were observed at 4.74 min and at 5.00 min retention times for standard and samples of ranolazine respectively. The precision (RSD = 0.2%) and accuracy (recovery = 100.4 – 100.5%) of the proposed method were found to be good. Lacosamide in pharmaceutical formulations was determined using Inert sustain HP C18 as stationary phase, a mixture of potassium dihydrogen phosphate (pH 2.0) and acetonitrile (90 : 10 v/v) as mobile phase A, ,mixture of acetonitrile and water (60 : 40 v/v) as mobile phase B. The method with high precision (RSD 0.04 %) and good accuracy (recovery 98.8 – 101.4 %) is useful for the determination of lacosamide in the range 0 to 150 μg mL-1. Both the methods were found to be highly robust.

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