UV-SPECTROPHOTOMETRIC ABSORBANCE CORRECTION METHODS FOR THE SIMULTANEOUS ESTIMATION OF PERINDOPRIL ARGININE AND AMLODIPINE BESYLATE IN BULK AND TABLET DOSAGE FORM
Murugan S.* and Vetrichelvan T.
ABSTRACT
Two UV-spectrophotometric methods have been developed and validated for simultaneous estimation of Perindopril arginine (PER) and Amlodipine besylate (AMD) in bulk and tablet dosage form. The Method A employs absorption correction method in zero order, the wavelengths selected were 229 nm for PER (but AMD shows absorbance) and 354 nm for AMD. Method B employs, absorption correction method in first order, the wavelengths selected were 204 nm for PER (but AMD shows absorbance) and 324 nm for AMD. For both the methods drugs were linear between the range of 5-25 μg/ml for PER and 6.925-34.675 μg/ml for AMD using methanol as solvent. The correlation coefficient was found to be 0.9994 for PER and 0.9992 for AMD for method A and 0.9996 for PER and 0.9999 for AMD for method B, respectively. The precision (intraday, interday) of methods was found within limits (RSD < 2%). It could be concluded from the results obtained in the present investigation that the two methods for simultaneous estimation of PER and AMD in bulk and tablet dosage form are simple, rapid, accurate, precise and economical and can be used, successfully, in the quality control of tablet formulations and other routine laboratory analysis.
Keywords: Perindopril arginine, Amlodipine besylate, Absorption Correction Method in Zero order, Absorption Correction Method in First order.
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