Abstract
INVESTIGATION OF THE PERFORMANCE OF BIPHASIC MEDIA IN THE CULTURE OF BRUCELLA

Noha A. Helmy* and Sally M. Abd Elsalam

ABSTRACT

Brucellosis is a common and significant bacterial disease among the world. Infected animals are generally detected by serological tests. Brucella culture and identification provide a definitive diagnosis but it needs several days. The aim of this study is to propose a biphasic media to cultivate Br. abortus S99for diagnostic and research purposes and to compare it with the glycerol potato agar OIE medium that is frequently used in routine preparing antigens and to assess the effectiveness in culturing Br. abortus S99. The culture obtained from biphasic media was evaluated microscopically, biochemically and molecularly .Rose Bengal antigens were prepared and compared with the one prepared by classical culture of Br. Abortus S99. All sera samples were tested by plate for all antigens prepared. The three antigens were prepared from biphasic media had the agreement with classical Rose Bengal and SAT. The biphasic media exhibited good growth of the strain in less time. The present study depicts the facilitate manipulation, low cost effective that help in cultivation of brucella without doing subculture and may use with limited expertise or equipments if all safety precautions are taken.

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