European Journal of Biomedical and Pharmaceutical Sciences

THE EFFECT OF SENGGANI LEAVES (Melastoma malabathricum L.) EXTRACTION METHOD ON TOTAL PHENOLIC LEVELS, TOTAL FLAVONOIDS, AND ANTIOXIDANT ACTIVITIES

Sri Luliana, Isnindar* and Tion Rusmawijayanto

ABSTRACT

Extraction method is important in maintaining the stability of the target compound. The purpose of this study was to observe the effect of the extraction method on total phenol levels, Total Flavonoids levels, and antioxidant activity. The extraction process used percolation and soxhletation methods with 96% ethanol as solvent. Preliminary tests of antioxidant activity were carried out by TLC with silica GF254 stationary phase and mobile phase using chloroform: methanol: ethyl acetate: water (80: 12: 6: 2). Positive results are characterized by pale yellow spots after being sprayed with 0.2% DPPH. Total phenol levels, Total Flavonoid levels, and antioxidant activity with the percolation method were 255.89±3.16 mg GAE/g sample; 36.92±0.22 mg QE/g sample; 16.00±1.76 μg/mL, respectively. Meanwhile, total phenol levels, Total Flavonoid levels and antioxidant activity with the the soxhletation method were 268.76±2.77 mg GAE/g sample; 41.01±0.35 mg QE/g sample; 15.13±2.67 μg/mL, respectively. In conclusion, extraction method affected the total phenol levels and Total Flavonoids levels (sig p <0.05), but not antioxidant activity (sig p> 0.05).

Keywords: Antioxidant, Melastoma malabathricum L., extraction method, DPPH.