European Journal of Biomedical and Pharmaceutical Sciences

PLASMID CURING POTENTIALS OF SOME MEDICINAL PLANTS AGAINST CITRATE NEGATIVE MOTILE SALMONELLA SPECIES

Iheukwumere I. H.*, Dimejesi S. A., Iheukwumere, C. M., Chude C. O., Egbe P. A., Nwaolisa C. N., Amutaigwe E. U., Nwakoby N. E., Egbuna C., Olisah M. C. and Ifemeje J. C.

ABSTRACT

Studies have shown that about 80% of multiple antibiotics genes, which possess threat to public health, are encoded in the plasmid. This study was undertaken to evaluate the plasmid curing potentials of Zingiber officinale (ZO), Ocimum gratissimum (GO) and Xylopia aethiopica (XA) extract against different strains of citrate-negative motile Salmonella species. Water samples were drawn from different water bodies closed to different hospitals, and screened for the presence of citrate-negative motile Salmonella species using pour plate technique. The isolates obtained were appropriately characterized. The resistance strains were detected by subjecting the isolates to antibiotic susceptibility test using disk diffusion technique. The plasmid curing potentials of the extract were ascertained by exposing the resistance isolates to different concentrations (1 %, 2 %, 3 %, 4 %, 5 %, 6 %, 7 % and 8 %) of the extracts, and subsequently to susceptibility test. The study revealed that 86 %, 71 %, 64 %, 55 % and 44 % of the citrate-negative motile Salmonella species (G, P, H, M and N) showed multiple antibiotics resistance to Ceporex (CEP), Ciprofloxacin (CPX), Ampicillin (PN), Septrin (STX) and Streptomycin (S). The multiple antibiotics resistance isolates were significantly (P<0.05) reduced by ZO (19 %, 12 %, 0 %, 0 %, 0 %), OG (33 %, 24 %, 7 %, 18 %, 0 %) and XA (43 %, 35 %, 29 %, 18 %, 11 %) extracts. From the above study, the extracts possessed pronounced plasmid curing potentials, of which ZO extract was most effective against the resistance strains.

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