GELATIN-ALGINATE SPONGE: A POTENTIAL SCAFFOLD FOR ADIPOSE TISSUE ENGINEERING
Hao Thi Thu Nguyen, Quan Minh To, Thao Duy Huynh, Toai Cong Tran, Ha Le Bao Tran*
ABSTRACT
Gelatin-alginate sponge (GA sponge) is one of the ideal materials to be studied and applied in soft tissue
regeneration, especially in adipose tissue engineering. This sponge is made from the combination of gelatin and
alginate with crosslinking activity of 1-Ethyl-(3-3-dimethylaminopropyl) carbodiimide. Gelatin and alginate were
mixed at ratio 8:2 with 0.3% EDC and freeze-dryed to form the GA sponge. After that, GA sponge was evaluated
the structure by Hematoxylin and Eosin staining method and scanning electron microscopy imaging, the
components by Fourier Transform Infra Red, the water absorption, in vitro biodegradation by using collagenase; in
vitro cytotoxicity towards human fibroblasts. Finally, adipogenic differentiation potential of human adiposederived
stem cells inside the GA sponge was studied. The results show that the GA sponge with spongy and stable
properties has high water absorption capacity, in vitro biodegradability, non-cytotoxicity. Simultaneously, human
adipose-derived stem cells can adhere and differentiate into fat cells within the GA sponge. With these results, the
GA sponge has properties suitable for applications in adipose tissue engineering.
Keywords: Gelatin, Alginate, sponge, scaffold, tissue engineering, adipose derived stem cells.
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