Abstract
EXTRACELLULAR LIPASE PROFILE OF STAPHYLOCOCCUS AUREUS ISOLATED FROM WOUND

*Orjiakor I. P., Olowomofe, T. O., Ajayi A. O., Fatoyinbo, A. A., Ukhureigbe O.M. and Femi-Ola, T.O.

ABSTRACT

Staphylococcus aureus has always been reported for the clinical importance in infection while the industrial potential can easily be overlooked; therefore this research was designed to study the extracellular lipase activity of Staphylococcus aureus isolated from surgical wound culture. Forty-one isolates of Staphylococcus sp. were collected, inoculated and incubated; the isolates were confirmed using cultural, morphological and biochemical characteristics. The lipolytic activity was determined qualitatively using Tributyrin agar. The lipase produced was further quantified. Twenty-six (63.4%) of the isolates fermented mannitol. The isolates of Staphylococcus aureus were coagulase and catalase positive. All the confirmed twenty-six isolates were also positive for lipase production by hydrolysing the media. The specific activities of lipase produced by the Staphylococcus aureus strain ranged from 0.008μm/min/mg to 0.050μm/min/mg. The Staphylococcus aureus strains reached their peak of lipase production at different time intervals. Twenty-seven percent of the isolate reached a peak of lipase production between 20-25hrs of incubation while twenty-three percent (23%) each reached peak between 10-15hrs and 15-20hrs, the next twelve percent (12%) reach their peak between 35-40hrs and approximately four percent (4%) attained their peak between 25-30hrs and 30-35hrs. The differences in the growth rate and attainment of peak of lipase production may be an indication that the Staphylococcus aureus varied widely in their physiological and biochemical properties.

Keywords: Enzyme, Lipase, Mannitol, Staphylococcus aureus


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tanima Debnath Sarkar

Download Article : Click here

News & Updation

  • EJBPS: OCTOBER ISSUE PUBLISHED

    OCTOBER 2021 Issue has been successfully launched on 1 October 2021

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT