European Journal of Biomedical and Pharmaceutical Sciences

ANTIBIOTIC RESISTANCE PATTERN OF BACTERIA ISOLATED FROM RECREATIONAL WATERS IN ADO-EKITI METROPOLIS

Oluyege J. O., *Orjiakor P. I., Olowomofe T. O., Anyanwu N. O., Ayannuga O. D. and Eze, C. N.

ABSTRACT

Antibiotic resistance patterns of bacteria isolated from Recreational waters in Ado-Ekiti metropolis were investigated in this study. Recreational waters were sampled before and after swimming. The visitors who came to swim were about an average of 4 per day. The Mean Total Bacterial Count was 3.49x102 cfu and the mean Coliform Count was 3.50x102cfu.The pH of the pool waters varied between a range of 6.50 – 7.30 over the sampling period. Pool turbidity range varied over a narrow range of 4.5 – 6.5 NTU, the nitrate range was 2.0 – 5.0 mg/L, while the magnesium hardness range was 20 – 60 mg/L respectively among other parameters. Four gram positive bacteria were isolated namely: Staphylococcus aureus, Staphylococcus epidemidis, Enterococcus sp., Enterococcus faecalis, while seven gram negative bacteria were isolated namely: Pseudomonas aeruginosa, Pseudomonas sp. Escherichia coli, Shigella typhii, Shigella dysenteriae, Klebsiella sp., Mycobacterium choloriae. 16 gram positive bacteria isolates including Staphylococcus aureus, Staphylococcus epidemidis, Enterococcus spp., Enterococcus faecalis were resistant to Pefloxacin, Gentamicin, Ampiclox, Cefuroxime, Amoxicillin, Rocephin, Ciprofloxacin, Streptomycin, Septrin and Erythromycin in the range of 4 – 100% resistant pattern, while 35 gram negative bacteria isolates including Pseudomonas aeruginosa, Pseudomonas spp. Escherichia coli, Shigella typhii, Shigella dysenteriae, Klebsiella spp., Mycobacterium choloriae were resistant to Ofloxacin, Reflacin, Ciprofloxacin, Augmengtin, Gentamicin, Streptomycin, Cephalexin, Naudixic acid, Septrin, Ampicillin in the range of 20 – 100% resistant pattern, some of the isolates were less resistant to Ciprofroxacin. The results of this study indicated that most of the swimming pools studied did not meet the WHO (2011) guidelines for drinking water. This can lead to serious public health implications since Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa which were present in the swimming pools pose health risk as swimmers may accidentally swallow some of the pool water.

Keywords: Antibiotics, Recreational Waters, Swimming pool, Coliform.