Abstract
INVESTIGATION OF PROTEIN RESISTANT SURFACES

*Nidiya Nubia Caleb, Atinga Vallada and Na’acha Frank Emina

ABSTRACT

In this study, we demonstrated the influence of size and shape of the model proteins (fibrinogen and bovine serum albumin) and surface chemistry on poly (KSPMA) brush and methyltriethoxy silane (MTEOS) on gold with respect to the adsorption kinetics. Adsorption profiles was built up for fibrinogen and bovine serum albumin (BSA) at concentrations of 31.2, 62.5, 125, 250, 500 and 1000 μg/mL, through manual injection over a period of 30 seconds for each concentration in that order into a quartz crystal microbalance (QCM) cell on gold, polymer 3-sulfonylpropyl methacarylate (KSPMA) brush and methyl-triethoxy silane (MTEOS). Substrate surfaces where fabricated by plasma enhanced chemical deposition (PECVD) using radiofrequency control at 3W with MTEOS, amino phenyl triethoxy silane (APTES) on QCM gold and Polymerisation of 3-sulfonylpropyl methacarylate (KSPMA) from α-Bromo-isopropyl bromide (BIBB) functionalised with APTES as initiator on gold quartz crystal. The amount of protein adsorbed onto poly (KSPMA) brush and MTEOS films was evaluated with quartz crystal microbalance: BSA was adsorbed more on the polymer (KSPMA) brush than on gold and conversely, fibrinogen was more on gold than the poly (KSPMA) brush. No protein adsorption was observed on MTEOS as result of the film thickness and super-hydrophobicity as seen with decreased frequency and increase bandwidth of the resonance spectra by 16MHz. This work shows that, size and shape of proteins determine their adsorption pattern on substrate surfaces. Also, the thickness of the film coating on the quartz crystal microbalance affects its sensitivity in detecting protein adsorption.

Keywords: Protein adsorption, Antifouling, Biomedical device, Biofouling and Surface chemistry.


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