European Journal of Biomedical and Pharmaceutical Sciences

ANTAGONISTIC ACTINOMYCETES FROM SOILS OF EAST GODAVARI DISTRICT, ANDHRA PRADESH, INDIA

*V. Sri. Venkateswara Rao, B. L. Prasanna, G. Sudhakar, K. Satish Babu, N. Madhu, P. Tharakeswara Rao, D. Konda Babu, T. Suresh

ABSTRACT

Actinomycetes are group of bacteria, which are present widely in ecological diversity. Actinomycetes are the major microbial population in soil which can produce active secondary metabolites. The aim of the present study is to isolate varieties of actinomycetes from different soil samples collected from different places in East Godavari district of Andhra Pradesh. All soil samples were appeared brown and black in colour and showed pH range between 7.0 to 7.6. A total of 16 actinomycete isolates were obtained from 4 different soil samples based on their colony morphology, mycelium colouration and diffusion of pigmentation. In the preliminary activity studies, four isolates exhibited good antibacterial activity by cross –streak method and 4 isolates exhibited good to excellent amylase and protease activities. It was concluded that isolate KAS1 had potential as source of new antibacterial compounds against Bacillus subtilis, Escherichia coli, Proteus vulgaries and Pseudomonas aeruginosa. Isolate KS4 showed maximum amylase and protease activity.

Keywords: Actinomycetes, secondary metabolites, Bacillus subtilis, Escherichia coli, Proteus vulgaries, Pseudomonas aeruginosa, amylase and protease activity.