European Journal of Biomedical and Pharmaceutical Sciences

DEVELOPMENT OF COST EFFECTIVE AND ECONOMIC METHOD FOR CULTIVATION OF CHLORELLA SINGULARIS USING WASTE CARBON AND NITROGEN SOURCE

Mahek Gupta* and Prachi

ABSTRACT

Single cell organism based oil from oleaginous microorganisms has involved the attention because of its significance in the production of biodiesel as well as reducing pollution and waste. This possibility of algal biomass for utilizing waste carbon and nitrogen content was tested in this study. In this work, Chlorella singularis was cultivated in medium containing waste carbon, nitrogen as a nutrient source and BBM medium as control. The growth kinetics of the algae on these mediums was observed. The microalgae, Chlorella singularis can utilize the nitrogen content present in biogas digester wastewater as a substrate for its growth. Different low cost and easily available carbon and nitrogen based feedstock such as Potato peel, and Pea peel was used in this study. The growth of microalgae was found to follow the Monod growth model satisfactorily. Under the optimum photobioreactor condition, a maximum biomass of 3.75 gm/l in waste carbon and nitrogen medium and 1.5 gm/l in BBM was obtained in fifteen days of batch process. Based on Mond growth kinetics, net specific growth rate and doubling time of Chlorella singularis was found to be 0.054 day-1 and 12.83 hr-1.

Keywords: Orange peel, Pea peel, Chlorella singularis, Monod kinetics, closed photo-biotector cultivation.