European Journal of Biomedical and Pharmaceutical Sciences

DEVELOPMENT, OPTIMIZATION AND VALIDATION OF UV VISIBAL SPECTROPHOTOMETRY – RECIVEW

Ashwini Chandewar* and Monika Jadhao

ABSTRACT

Ultraviolet (UV) spectroscopy is a physical technique of the optical spectroscopy that uses light in the visible, ultraviolet, and near infrared ranges. The Beer-Lambert law states that the absorbance of a solution is directly proportional to the concentration of the absorbing species in the solution and the path length. Thus, for a fixed path length, UV/VIS spectroscopy can be used to determine the concentration of the absorber in a solution. It is necessary to know how rapidly the absorbance changes with concentration.[UV-VIS Spectroscopy is the term used for the analytical evaluation of the different types of the solvents and substances. Spectroscopy is generally preferred especially by small-scale industries as the cost of the equipment is less and the maintenance problems are minimal. The method of analysis is based on measuring the absorption of a monochromatic light by colorless compounds in the near ultraviolet path of a spectrum (200-400nm). The pharmaceutical analysis comprises the procedure necessary to determine the “identity, strength, quality and purity” of such compounds. It also includes the analysis of raw material and intermediates during the manufacturing process of drugs. It is well known that the dissociation constant is a most important parameter in development and optimization of a new compound for effective formulation development.

Keywords: UV- VIS Spectroscopy, Optimization Techniques, Electrochemical methods.