European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF PHYTOCHEMICALS SCREENING AND ANTIOXIDANT ACTIVITY OF VITIS VINIFERA (GRAPES) FRUIT EXTRACT USING FENTON REACTION

Wasim Raja*, Amit Dubey and Pratibha Verma

ABSTRACT

Grapes (Vitis vinifera L.) are a major fruit crop in the world. Grapes seem to confer health benefits due to their antioxidant activity. In traditional medicine system it is used especially for anticancer, blood purification, and as an antispasmodic. This plant was also useful in the health benefits associated with natural compounds and have been demonstrated with the emphasis on antioxidants. We have evaluated the phytochemical analysis and antioxidant potential of selected grape variety from India. The phenolic compound in fruits, vegetables, herbs and spices possess potent antioxidant, anti-inflammatory, antimutagenic and anticarcinogenic activities. The present study focused on the phytochemical screening and antioxidant activity of Vitis vinifera fruit Extract in vitro conditions. The berries of Vitis vinifera was extracted with methanol using a Soxhlet extractor. Phytochemical screening of the methanol extract of Vitis vinifera showed the presence of glycosides, phytosterol, saponins, tannins, flavonoids and terpenoids. The total phenolics content of leaf as determined by Fenton reaction and was found to be good antioxidant activity as different dose concentrations. The antioxidant activity of extract was carried out with ascorbic acid as a standard reducing agent. All the analysis was made with the use of UV-Visible Spectrophotometer. In this plant Vitis vinifera Extract there was a remarkable concentration dependent free radical scavenging and reducing power was exhibited. These findings demonstrated that Vitis vinifera Extract possess free radical and hydroxyl radical scavenging activity as well as antioxidant activity in vitro. In conclusion the present Study indicates that Vitis vinifera Extract may be a potential source of natural antioxidant. The results suggested that Vitis vinifera Extract could serve as a potential source of natural phytochemicals and antioxidant and can be used in any preparations for combating free radical mediated damage to the body.

Keywords: Antioxidant activity, Phytochemicals, Fenton Reaction, Hydroxyl radical, ascorbic acid, Vitis vinifera, TBARS.