Abstract
IN VITRO ANTIOXIDANT ACTIVITY OF ASPARAGUS RACEMOSUS LINN LEAVES: EFFECT OF EXTRACTION SOLVENTS

Tanaya Ghosh and Prasanta Kumar Mitra*

ABSTRACT

In vitro antioxidant activity of Asparagus racemosus Linn (A. racemosus L.) is known in literature. But there is controversy on the use of solvent by which maximum antioxidant activity can be obtained. Aim of the present study was, therefore, to see the effect of different extraction solvents on in vitro antioxidant activity of A. racemosus L. leaves. A. racemosus L. leaves were collected from the medicinal plant garden of the North Bengal University and identified by the taxonomist. Solvent extractions of the leaves were made separately by using chloroform, petroleum ether, methanol, ethanol, ethyl acetate and acetone. Extracts were separately dried and processed for in vitro antioxidant activity through superoxide anion generation by xanthine-/xanthine oxidase assay, linoleic acid peroxidation assay and by DPPH photometric assay. Quercetin was used as standard antioxidant. Results showed that ethanol extract of A. racemosus L. leaves had maximum antioxidant activity against the methods used which was comparable to that of quercetin.

Keywords: Asparagus racemosus linn. Leaves, solvent extractions, xanthine-/xanthine oxidase assay, linoleic acid peroxidation assay, DPPH photometric assay.


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