DETERMINING ABILITY OF ASTAXANTHIN EXTRACTED FROM HAEMATOCOCCUS PLUVIALIS ON STRESS-INDUCED HUMAN FIBROBLAST
Quan To Minh*, Nhan Tran Dinh, Dieu Tran Quang, Huy Nguyen Dinh Huy, Truc Vo Thi Thanh, Thao Huynh Thu and Bien Lai Dinh
ABSTRACT
Astaxanthin (ASX) is one of the powerful antioxidants, which has been highly effective against oxidative species. However, the use of this natural pigment in topical skincare products in the protection of skin from oxidative stress has yet to understand thoroughly. This study concerns the assessment of ASX on protecting human dermal fibroblast (hF) against H2O2. ASX was extracted from Haematococcus pluvialis using Sacha Inchi oil (eASX) and concentration determined by HPLC was 240.6 ± 21.11 μg/ml. hF was pre-treated with eASX (0.1 and 0.5 μg/ml) or commercial astaxanthin (comASX) for 24 hours before being senescence induced by incubating with 175 μM H2O2 for 90 minutes. In comparison to the control group, ASX groups showed the smaller cell nucleus area and lower senescence β-galactosidase (SA b-gal) expression. The cell nucleus area of hF in control group, eASX groups with the concentration of 0.1 and 0.5 μg/ml and comASX group were 305.5 ± 29.5μm², 281.8 ± 31.2 μm, 276.5 ± 38.1 μm² and 270.6 ± 26.9 μm², respectively. The percentage of SA b-gal-expressed hF in these groups was 58.9 ± 3.5%, 31.6 ± 4.6%, 29.4 ± 2.1% and 28.2 ± 3.9%, respectively. In conclusion, eASX has been shown to have the protection effective on hF against the senescence effect of H2O2.
Keywords: antioxidants, Astaxanthin, Haematococcus pluvialis, hydrogen peroxide.
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