European Journal of Biomedical and Pharmaceutical Sciences

STUDIES ON LEVELS OF SOME COLORECTAL CANCER ASSOCIATED ANTIGENS/MARKERS IN NEWLY DIAGNOSED COLORECTAL CANCER PATIENTS WITH DIFFERENT ABO BLOOD GROUPS IN YENAGOA

*Umeh Obadiah Ndudi, Dr. Nwadike N. Constance and Dr. Nnodim Johnkennedy

ABSTRACT

The study on levels of some colorectal cancer associated antigens/markers in newly diagnosed colorectal cancer patients with different ABO blood group was carried out at Federal Medical Centre, Yenagoa. A total of one hundred subjects with different ABO blood groups comprising fifty newly diagnosed colorectal cancer patients (16 of group A, 20 of group B and 14 of group O) and fifty control subjects (17 of group A, 18 of group B and 15 of group O), within the age bracket of 40-70 years were recruited for the study. Samples of blood were collected, prepared by standard methods and sera stored at -20ºc prior to use. Analysis was done using immunoturbidimetric assay, enzyme linked immunosorbent assay (ELISA) and gas chromatography-mass spectrometry techniques. Data obtained were subjected to statistical analysis using one way analysis of variance (ANOVA). The results obtained showed that there was a significant increase (p<0.05) in CEA value of male CRC group A, female CRC group A, male CRC group B, female CRC group B, male CRC group O and female CRC group O when compared to their control subjects respectively. The Butyric acid of male CRC group A, female CRC group A, male CRC group B, female CRC group B, male CRC group O and female CRC group O decreased significantly (p<0.05) when compared to their control subjects respectively. There was a significant decrease (p<0.05) in Propionic acid of male CRC group A, female CRC group A, male CRC group B, female CRC group B, male CRC group O and female CRC group O when compared to their control subjects respectively. A significant decrease (p<0.05) was observed in Interleukin-6 of male CRC group A and female CRC group A when compared to their control subjects. The Interleukin-8 showed a significant difference (p<0.05) in male CRC of both group B and O when compared to their control subjects. The patients of blood group B have the highest value in CEA followed by blood group O and blood group A patients respectively. Similarly, it was observed that patients with blood group B have the lowest values in short chain fatty acids followed by blood group O and blood group A patients. This could predict a more survival rate for colorectal cancer patients with blood group A and less survival rate for blood group B colorectal cancer patients. Therefore, this study concludes that there are observable changes in the CEA and short chain fatty acids of newly diagnosed CRC patients with different ABO blood groups attending Federal Medical Centre, Yenagoa.

Keywords: Colorectal Cancer, ABO blood group, Interleukin, Carcinoembryonic Antigen, Short Chain Fatty Acids.