PHYTOASSISTED SYNTHESIZED COPPER NANOPARTICLES FROM FICUS KRISHNAE FOR IT’S ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY
Amarvani P. Kanjikar and Ramesh L. Londonkar*
ABSTRACT
Today the use of copper in different fields of science is due to its properties such as high conductive, good potential reduction and more economic compared to silver and good. Based upon recent survey of research articles on cupper nanoparticle shows potential prevention of infection and antimicrobial agent. In this paper, the copper nanoparticle has been synthesized based on green synthesis method using Ficus krishane stem bark aqueous extract without external and capping energy. It was characterized by UV-Visible spectrophotometry, X-ray diffraction and transmission electron microscopy analysis. Copper nanopartcles formation was recognized by colour changes from dark brown and was further proven by UV-visible spectrophotometer analysis based upon the absorption of band at 350-370 nm. The XRD photograph indicated that the face-centered cubic structure of the synthesized CuNPs. SEM study analyzed that the size from 160-228 nm with interparticle distance, whereas shape is spherical. X-ray Diffraction pattern performed three sharp peaks specifically referred to face-centered cubic structured of metallic copper. The antibacterial activity of copper nanoparticles was studied against disease causing pathogens like Staphylococcus aureus and Enterococcus fecalis. Antioxidant activity of copper nanoparticle by DPPH method was carried out. The present study confirms that the biosynthesized CuNPs from Ficus krishnae stem bark extract have a great affiance as antibacterial and antioxidant agent.
Keywords: Ficus krishnae, Copper nanoparticles, XRD, SEM, UV-visible spectroscopy and antibacterial activity.
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