COLORIMETRIC DETERMINATION OF MESALAMINE IN PURE AND DOSAGE FORMS
Safaa Mohamed Abdel Hamide* and Nagat A. Abbas*
ABSTRACT
Three simple, sensitive, economic, rapid and reproducible spectrophotometric methods I, II, III are described for
determination of mesalamine in pure drug as well as in tablet dosage forms. Method I is based on the reaction of
mesalamine with vanillin in phosphate buffer medium with pH equals 7, forming yellow colored Schiff s base
complex with absorption maximum at 380 nm. Method II describes, the reaction of mesalamine with ninhydrin in
dimethylformamide forming pink colored complex with absorption maximum at 550 nm. Method III is based on
the reaction of mesalamine with P-benzoquinone giving yellow colored complex with absorption maximum at 390
nm, under optimum conditions, mesalamine could be quantified in the concentration range of (100-400)μg/ml,
(50-300)μg/ml and (70-300)μg/ml for methods I, II and III ,respectively the correlation coefficients are 0.9999,
0.9998 and 0.9995 for methods I, II and III respectively which show the linearity of the response against
concentration. The methods were validated as per ICH guidelines. The developed and validated colorimetric
methods are suitable for assay determination in pure and pharmaceutical formations which are more useful with
respect to less analysis time.
Keywords: Spectrophotometry, mesalamine, vanillin, ninhydrin, P-benzoquinone, validation.
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