European Journal of Biomedical and Pharmaceutical Sciences

SENSITIVITY STUDY WITH RESPECT TO MATRIX EFFECT IN BIO-ANALTICAL STUDIES -A CRITICAL REVIEW

Adiba Sahiya*, Imam Pasha Syed, Anupama Koneru and M. Mushraff Ali Khan

ABSTRACT

In this critical review authors presented effect of matrix effect in sensitivity ad reproducibility of analytical methods for quantitative estimation of biological samples. In bio analytical method effect of different matrix can cause decrease in sensitivity and affect the assay result and reproducibility. For procedures using matrix in method development it is mandatory to evaluate and quantify matrix effect. According to regulatory guidelines, in validation determination of matrix effect is essential. Matrix effect is defined as the effect of co-eluting residual matrix component of biological sample on the ionization of target. There are two types of the matrix effect absolute matrix effect and relative matrix effect .Two types of absolute matrix effect are ion suppression and ion enhancement. Matrix effect may cause variation in sensitivity with respect to time, inaccuracy of result and chromatographic peak variation. Protocol specifications of matrix reduction should be compatible with analysis. Methods for sample clean up include liquid extraction and solid phase extraction. Matrix effect can be caused by both organic and inorganic substances. Matrix effect is often determined by post column infusion and post extraction spiking methods.

Keywords: Matrix effect, Biological sample, Analysis, LC-MS/MS, Sensitivity.