GREEN SYNTHESIS, CHARACTERIZATION AND PHARMACOLOGICAL ACTIVITIES OF SILVER NANOPARTICLES PREPARED FROM PTEROCARPUS MARSUPIUM
Dr. Aruna L. Hugar and Prof. Ramesh L. Londonkar*
ABSTRACT
Background: Green synthesis of silver nanoparticles has gained much interest among the scientific community because of their colossal applications. In this concern, Indian flora has yet to divulge innumerable sources of cost effective, ecofriendly compounds in preparing AgNPs. Consequently, the present study involves an efficient and sustainable route of AgNPs preparation using P.marsupium bark extract which is well adorned for their medicinal properties. Objective: This study aims to investigate characterization and evaluation of silver nanoparticles prepared by green synthesis method using P.marsupium bark. Methodology: The green synthesis of silver nanoparticles from P.marsupium bark extract was accomplished. Synthesized AgNPs were characterized by UV-Vis spectrophotometer, SEM, XRD and FTIR analysis. The antibacterial efficacy was determined by disc diffusion method and antioxidant activity by DPPH method. In vitro cytotoxicity effect of biosynthesized AgNPs in SKOV-3 ovarian cancer cell line by MTT assay was also carried out. Results: The UV-VIS spectrum of silver nanoparticles revealed a characteristic Surface Plasmon Resonance (SPR) peak at 435nm. SEM study showed a spherical shaped AgNPs and size of AgNPs were about 240nm. XRD photograph indicated AgNPs were in crystalline face-centered cubic structure and the size range of 15-30 nm, further confirming the result of SEM. FTIR result also showed that the synthesized nanoparticles were capped with bimolecular compounds which are responsible for the reduction of silver ions. The AgNPs nanoparticles have shown a potential antibacterial, antioxidant and cytotoxicity activities when compared with their respective standards. Conclusion: The present study confirms that the biosynthesized AgNPs from P.marsupium bark extract were found to be ecofriendly, easy, cost effective and have promising pharmacological activities.
Keywords: P.marsupium, silver nanoparticles, characterization, disc diffusion assay, DPPH, SKOV-3 cells.
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