Abstract
ANALYSIS OF INSULIN IN PHARMACEUTICAL PREPARATIONS PRESENT IN LIBYAN COMMUNITY PHARMACIES

Hamed Jamal Ben Alhaj, Abdulrahman Faraj Beleed, Moaad Al-Mahdi Juhaider, Zaema El-Baroudi, Amal K. Belaid, Asma M. Eswayah and Ruwida M. Kamour*

ABSTRACT

Background: Insulin is an anti-diabetic drug which is used for the treatment of diabetes type I. There are many methods for analyzing insulin starting from ELIZA to HPLC methods. There are many brands for insulin preparations that are imported by private companies in addition to those provided by the National Hospital of Diabetes. Aim: This study aims to analyze random samples of insulin preparations collected from different community pharmacies in Tripoli-Libya in an attempt to check if these brands have adequate insulin concentrations for diabetic patients. Method: In this study, random samples were collected for insulin preparations in the form of insulin pens. They were analyzed using spectroscopic method. An absorption maximum was found to be at 276 nm using 0.01M HCl as a solvent. The results were compared to that of similar samples obtained from the national hospital of diabetes. Results: The decrease in amount of insulin in the later two brands reached 10% or more making them inappropriate to deliver the needed amount of insulin at different doses. This is very vital because it will lead to serious problems on short as well as long terms to diabetic patients.

Keywords: Insulin, spectrophotometric analysis, absorbance and dose.


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