Abstract
ANTIMICROBIAL ACTIVITY OF LEAVES OF THUJA COMPACTA AGAINST PATHOGENIC ORGANISMS COMPARED WITH CONTROL DRUGS

Aishwarya N. Kapse* and C. J. Chandekar

ABSTRACT

Fresh juice and acetone, chloroform, methanol, petroleum ether and water extracts of leaves of Thuja compacta traditionally used in Indian system of medicines were screened against standard strains of the Escherichia coli NCIM 2931, Salmonella typhi MTCC 734, Salmonella typhimurium MTCC 98, Klebsiella pneumoniae MTCC432, Proteus vulgaris NCIM2857, Proteus mirabilis MTCC425, Pseudomonas aeruginosa NCIM5029, Staphylococcus aureus MTCC 96, Staphylococcus epidermis MTCC 435, Bacillus cereus NCIM 2155, Bacillus subtilis NCIM 2063 and Bacillus megaterium NCIM 2087 by using agar well diffusion method. Fresh leaves juice showed significant activity against Gram negative organisms except E. coli and S. typhi. Similarly active against Gram positive bacteria except S. aureus, S. epidermis. Acetone, chloroform, methanol, petroleum ether extracts of leaves showed significant activity against only Gram positive bacteria. Zone of inhibition of the extract compared with the standard antibiotics, Am.[30] Amoxycilin; Cf.[30] Ciprofloxacin; Co.[25] Cotrimaxazole; G.[50] Gentamicin and Tetracycline—T.[30] It is concluded that the Thuja compacta may serve as valuable source of compounds with therapeutic potential.

Keywords: Antimicrobial activity, Thuja compacta, Antibiotics, Acetone, Chloroform, Methanol and Petroleum ether extracts.


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