European Journal of Biomedical and Pharmaceutical Sciences

DRUG STANDARDIZATION AND MONOGRAPHS DEVELOPMENT, HPTLC. FINGER PRINTING RESEARCH STUDIES OF POLYHERBAL FORMULATION - HABB-E-NISHAT JADEED

*Pawan Kumar Sagar, R. Murugeswaran, Rampratap Meena, Asma Sattar Khan, S. Mageswari, P. Meera Devi Sri, Sonali Sajwan and Syed Gulnawaz Ahmad

ABSTRACT

Standardization is used to describe all measures under taken during the manufacturing process and quality control of drug leading to its reproducible quality. Most of the traditional medicine are effective but still they lack in its standardization. Therefore we need to develop standard techniques to standardize and validate of the herbal formulations. The drug Habb-e-Nishat Jadeed is therapeutically useful in the treatment of Zof-e-Bah (Sex debility). The drug Habb-e-Nishat Jadeed was prepared in three different batches as per the guidelines of National Formulary of Unani Medicine(Part-VI). Present study is aimed to evaluate the pharmacopoeial standards using physico-chemical parameters; HPTLC fingerprinting, quality control and assurance parameters using WHO guideline to ascertain the quality of drug. The physico-chemical data showed that the drug contain LOD/moisture (2.33%), total ash (7.7%), acid in-soluble (4.32%), alcohol and water soluble extractive matter (20.39%) & (23.10%), pH (1% solution), (5.59), pH (10% solution) (5.66), ASS Extractive (16.13%) and CSS Extractive (7.86%), Bulk density of granules(0.5467) and the TLC/HPTLC finger prints showed various spots at 254nm, 366nm and visible light (V-S reagent). The quality control study revealed the absence of microbial load, aflatoxins, heavy metal and pesticide residues. The evaluated standards will be very useful for laying the pharmacopoeial standards of Habb-e-Nishat Jadeed and also in providing the quality medicine to needful human being.

Keywords: Habb-e-Nishat Jadeed, Physico-chemical quality, Quality Control and Assurance parameters, TLC/ HPTLC fingerprinting and Unani Compound drug.