Abstract
TOTAL PHENOLICS, FLAVONOID, ASCORBIC ACID, AND IN-VITRO ANTIOXIDANT ACTIVITY OF SOYA CHEESE

Ummey Hafsa Bithi, M. N. Uddin and Kanika Mitra*

ABSTRACT

In this work, the contents and antioxidant ability of various classes of phenolic compounds present in the Cheese of soybean were evaluated. Total phenolics and flavonoids were determined spectrophotometrically by using tannic acid and catechin standards, respectively after extraction of Cheese with methanol. Also, the ascorbic acid contents were determined by UPLC-MS-MS.The in vitro antioxidant models studied are the total antioxidant capacity by phosphomolybdenum method, the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay, and reducing power by using standard ascorbic acid. The methanol solvent extract of cheese contains significant amounts of phytochemicals including total phenolic (123.3 mg gallic acid equivalent/100g), and flavonoids (78.28 mg catechin equivalents/g) compared to the raw soy extract. Similarly, in the cheese extract of methanol solvent, has high level of total antioxidant activity (about 472.07 mg ascorbic acid equivalent/100g) and reducing power (11.76±0.09 mg ascorbic acid equivalent per 100 gm) than raw soy. The quantity of ascorbic acid was found to be 119.03±4.07μg/100g in Cheese. The free radical scavenging activity of the Cheese was measured and the results are shown that the IC50 is 138.73±3.00mg (fresh basis) and 43.01±0.92 mg (dry basis). The results of the present investigation strongly indicate the importance of this soy product for human health.

Keywords: UPLC-MS-MS, ascorbic acid, total phenols; flavonoids; total antioxidant activity; DPPH assay; reducing power.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • EJBPS: JANUARY ISSUE PUBLISHED

    JANUARY 2020 Issue has been successfully launched on 1 January 2020.

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT