European Journal of Biomedical and Pharmaceutical Sciences

IN-VITRO ANTIDIABETIC INVESTIGATION OF PSOPHOCARPUS TETRAGONALOBUS HYDROALCOHOLIC POD EXTRACT

Dhanya Rajan E. P.*, Rakesh K. Jat and Sujith S. Nair

ABSTRACT

Medicinally important herbal plants are one of the primary assets of remedial specialists. Undoubtedly, 80% of the total populace utilizes plants in medical care. There is an expanding interest in utilizing therapeutic plants and their phytoconstituents as normal sources due to their notable pharmacological effects in the body. Psophocarpus tetragonalobus of the Fabaceae commonly known as winged bean is well known for its high nutritional value but remains as an underutilized crop. The intention of this study was to explore the scientific basis of traditional usage of Psophocarpus tetragonalobus as an anti-diabetic agent in hyperglycemic patients. Hydroalcoholic extract from its dried pods were prepared and were analyzed both qualitatively and quantitatively for evaluation of phytochemical agents followed by anti-diabetic study in vitro.The results of phytochemical investigation indicated the presence of carbohydrates, proteins, phenols, flavonoids, glycosides, amino acids and fatty acids in crude extract. The prepared hydroalcoholic extract of the pods were analysed for its α-amylase and alpha glucosidase inhibition assay using the 3, 5- dinitrosalicylic acid method. The IC50 values of α amylase and α- glucosidase inhibitory activity of PTPH were 722.65±0.29 and 302.47 ±0.34 respectively and were closer to that of the standard drug Acarbose which exhibited the IC 50 values of 548.74±0.38 and 231.22 ±0.93 respectively. The pod extracts of P.tetragonalobus exhibit notable α-amylase and glucosidase inhibitory activity in the crude hydroalcoholic extract and hence can be used as a regular green vegetable and also be investigated further in isolating pure compounds with anti-diabetic activity.

Keywords: Psophocarpus tetragonalobus, anti-diabetic, hydroalcoholic extract, Amylase, Glucosidase