Abstract
IMPACT OF ACORUS CALAMUS AND FERULA ASAFOETIDA IN ANTI SNAKE ENVENOMATION STUDIES-A PHYTOCHEMICAL, SPECTROSCOPIC AND CHROMATOGRAPHIC PERSPECTIVE

Anoop M.*, Haritha Venugopal and Venugopal K. G.

ABSTRACT

The scheme of examining the bioactive components in Acorus calamus and Ferula asafoetida plants alone and in combination with other plants were presented in this work that extrapolates the idea behind traditional anti snake envenomation practices using the selected medicinal plants through a preliminary piece of phytochemical ,chromatographic and spectroscopic analysis. The Rf values depicted in the ranges of 0.9 and 0.75, 0.645 for acetone extracts of Acorus calamus and Ferula asafoetida respectively that indicated the presence of a component that can stimulate the anti venom approach. The UV-VIS spectroscopic bands clearly represented presence of terpenoid class of compounds in the ranges specially in 200-320 nm for Ferula asafoetida acetone extracts and 500-700 nm ranges for the aqueous extract of Acorus calamus. The dispense of brown color ring formation stipulated the existence of terpenoid classes as secondary metabolite. These preliminary assays extended the way for further future interpretations of the residence of active principles that may be assayed through FTIR and mass spectrometer studies. The work aimed a Homoeopathic formulation that may be triggered through an ultradilution technique and nanoparticulate formulations from a purified bioactive extract that may be a constructive approach in traditional snake envenomation management practices.

Keywords: Acorus calamus, Ferula asafoetida, TLC, Phytochemical, Spectroscopic Homoeopathy.


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