European Journal of Biomedical and Pharmaceutical Sciences

PHARMACOGNOSTICAL, PHYSICO-CHEMICAL AND HPTLC PROFILE OF SHALGUM SEED (BRASSICA RAPA L.)

A. Jaculin Raiza*, R. Murugeswaran, S. Mageswari, P. Meera Devi Sri, Rampratap Meena and N. Zaheer Ahmed

ABSTRACT

The main objective of the present study was carried out to determine the pharmacognostical, phytochemical, physicochemical and HPTLC characteristics of Unani single drug shalgum seeds (Brassica rapa L.) The phytochemical screening reveals the presence of alkaloids, flavonoids, tannins, glycosides and phenolic compounds in alcohol, chloroform and aqueous extracts. The physicochemical data showed that the drug contained foreign matter, moisture, total ash, acid-insoluble ash, extraction values, pH and volatile oil contents. The finger print analysis was carried out using High Performance Thin Layer Chromatography (HPTLC) methods for the separation of the active constituents in extracts. Alcohol and chloroform extractives of seeds showed various spots at 254 and 366nm (UV region) and revealed the presence of peaks with Rf values. In pharmacognostical studies the macroscopic, microscopic and powder features of shalgum seeds were carried out. The seeds were also analyzed for microbial load and for specific pathogenic bacteria as per WHO norms respectively.

Keywords: Shalgum; Brassica rapa L.; HPTLC; Phyto-chemical screening, Physicochemical parameters; Pharmacognosy;