European Journal of Biomedical and Pharmaceutical Sciences

STUDY OF THE BIOLOGICAL EFFECTS OF AN ESSENTIAL OIL PROCESSED FROM LEAVES OF EUGENIA ASTRINGENS CAMBESS SPECIMENS. (MYRTACEAE)

Santos, J.L.; Cruz, G.C.S.; Nascimento, C. C. H.C.; Camacho, A. C. L. F.; Oliveira, J. F. F.; Gomes, M.L.; Azevedo, L.A.C.; Pinto, P. R.; Nascimento, S. F.; Vasconcelos, S.D. D. de; Nogueira, R. I.; *Barreto, A. S.; Diré, G. F.

ABSTRACT

The essential oil (EO) from the leaves of Eugenia astringens Cambess. It was obtained by hydrodistillation using Clevenger apparatus modified. Essential oil chemical composition was analyzed by GC and GC / MS, which showed a yield of 0.17%. The major component was found to be the α -pinene. The result of quantification of the EO sample, the calibration curve showed that the percentage of α -pinene is present in the essential oil of 77%. Cytotoxic potential and genotoxic of the essential oil of E. astringens Cambess. was assessed in vitro, by indirect diffusion in agarose gel in a lineage of Staphylococcus aureus. In the cytotoxic test, the essential oil obtained have antibacterial activity in vitro relevant for high dosage (25 μL), and the combination of EO associated with the amoxicilina, both in the dosage of 12.5 μL, was observed that antibiotic action was not potentiated. The rating of genotoxic test associated with H2O2 and stannous chloride (SnCl2) showed that with 12.5 μL the essential oil potentiated the genotoxic effect of SnCl2. In the dosage of 8.5 μL, OE showed genotoxity action when tested with the composts cited. There were cytotoxic and genotoxic tests with the α – pinene, under the same conditions EO, in order to compare the effectiveness of both.

Keywords: Eugenia. GC/MS. Cytotoxic. Genotoxic. ? – pinene.