Abstract
CHARACTERISATION OF SYNTHESIS OF SILVER NANOPARTICLES FROM BARK EXTRACT OF BUTEA MONOSPERMA VAR. LUTEA AND THEIR ANTICANCER ACTIVITY ON HELA CELL LINE

Vibha Bhingradiya and Nainesh Modi*

ABSTRACT

Butea monosperma var. lutea is commonly known as the yellow flame of the forest. It is a medium-sized dry season-deciduous tree growing to 49 ft tall but taller than well-known Butea monosperma. The synthesis of AgNPs using bark extract of Butea monosperma var. lutea, which is used at room temperature as both a reducing and capping agent. The mixture for the reaction turned brownish yellow after about 24 h and an intense surface plasmon resonance (SPR) band at around 406 nm clearly indicates the formation of silver nanoparticles. The presence of stabilizing silver nanoparticles is shown by a UV – Visible spectrophotometry. For nanoparticles, surface. Fourier Transform-Infrared (FT-IR) spectroscopy showed that the nanoparticles were capped with the compounds present in the plant extract. Moreover, these biologically synthesized nanoparticles have also shown an outstanding cytotoxic impact on HeLa cells line by MTT assay.

Keywords: Silver Nanoparticles, Bark Extract, UV – Visible Spectroscopy, FTIR, HeLa Cell line, MTT


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