European Journal of Biomedical and Pharmaceutical Sciences

A REVIEW: MASS SPECTROMETRY HYPHENATIONS FOR THE COMMON SEPARATION TECHNIQUES, THEIR INSTRUMENTATION AND ITS DIVERSE APPLICATIONS.

T. Sudha*, Srinivasan S. and S. Tamizhselvi

ABSTRACT

Many Traditional methods which involves single analytical techniques like HPLC, HPTLC etc. has become insufficient in order to carry out for analysis of large amount of compounds with accurate sensitivity as well as specificity. To overcome this type of difficulty in analysis hyphenated techniques in the year 1980 was introduced which mainly involved the coupling of spectroscopic technique and separation technique with the help of proper interface. In this review paper hyphenated MS techniques their instrumentation, Interfaces and applications were discussed for the commonly used separation techniques HPLC and HPTLC which have revolutionised in the field of analysis. Usage of HPLC-MS, HPTLC-MS is been increasing speeding day by day which has shown better analysis of sample.

Keywords: Hyphenated technique, HPLC-MS, HPTLC-MS, Interfaces.