HIGH NUMBERS OF MYELOID DERIVED SUPPRESSOR CELLS IN THEIR PERIPHERAL BLOOD OF METASTATIC BREAST CANCER PATIENTS
Aya Samir Farahat, Fatma Mohktar Fouda Mohamed Abd El-Hamid Alm El-Din, and *Mohamed Labib Salem
ABSTRACT
Background: Breast cancer escapes from anti-tumor immunity through several mechanisms, including emergence of immunosuppressive cellular mechanism such as myeloid-derived suppressor cells (MDSCs) which analyzed by flow cytometry. MDSCs have been found to include two subpopulations, the monocytic one which is more suppressive than the granulocytic one. Aim: the aim of this prospective study was to delineate the role of monocytic myeloid derived suppressor cells (Mo-MDSCs) and granulocytic myeloid derived suppressor cells (Gr-MDSCs) in patients with metastatic breast cancer (MBC) Subjects and Methods: This study included sixteen metastatic breast cancer in patients before and after chemotherapy and five healthy control volunteers. Flow cytometry was used to analyze the numbers and phenotypes of MDSCs (CD33+,CD11b+,CD14+andHLAD-R). Results: We found Significant increases in the relative and the absolute numbers of MDSCs after chemotherapy in the peripheral blood of metastatic breast cancer (patients than in the control group; (9.3 ± 2.03 vs. 1.2± 0.08, P < 0.003). Interestingly, the number of monocytic MDSC was higher than those of granulocytic MDSC;(6.7±0.5 vs 3.03±0.7). Conclusion: These data shed a light on one of the extrinsic cellular mechanism (MDSC) in metastatic breast cancer patients which might explain the escaping mechanism.
Keywords: Breast cancer; Metastatic; Myeloid derived suppressive cells; Monocytic MDSC; Patients.
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