European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF THERAPEUTIC POTENTIAL OF CLEOME VISCOSA ON CORTICOSTERONE INDUCED EXPERIMENTAL DEMENTIA IN MICE

Tripti Joshi*, Neeraj Kumar and Preeti Kothiyal

ABSTRACT

The present study was aimed to assess the effect of Cleome viscosa extract on learning and memory, brain cholinesterase levels and the oxidative stress markers in corticosterone treated mice. Mice weighing about 25-30 g were randomized into 12 groups. The extract was administered orally in three doses (100, 200 and 400mg/kg) for a period of 21 days, 30 minutes before corticosterone injection. Piracetam (200mg/kg i.p.) was used as a standard drug. Corticosterone in a dose of 5mg/kg was administered subcutaneously for 21 days. Behavior models, elevated plus maze and Morris water maze were used to assess the cognitive functions. Effect of extract on biochemical parameters (brain cholinesterase level, lipid per oxidation, superoxide dismutase and reduced glutathione) was also assessed in the brain tissue of mice. Corticosterone treated mice showed significant impairment in acquisition and retention as compare to vehicle control group. Pre treatments with Cleome viscosa extract for 21 days showed significant decrease in transfer latency in elevated plus maze and increase in time spent in target quadrant in Morris water maze compared to the negative control. Corticosterone cause significant increase in brain cholinesterase level, level of lipid per oxidation and reduce the level of antioxidant enzymes, superoxide dismutase and glutathione. Pre treatment with Cleome visocsa extract resulted significant decrease in brain cholinesterase level and lipid per oxidation and increase in the reduced glutathione and superoxide dismutase levels as compared to negative control. These results suggest that plant have a potential role in the management of cognitive dysfunctions.

Keywords: Alzheimer’s disease, dementia, elevated plus maze, morris water maze, lipid per oxidation, glutathione, superoxide dismutase.