European Journal of Biomedical and Pharmaceutical Sciences

CYTOTOXICITY EVALUATION OF PROSOPIS CINERARIA (L) DRUCE AGAINST BRINE SHRIMP

P.V.V. Satish, K. Somaiah, P. Brahmam, N. Sree Rekha and K. Sunita*

ABSTRACT

The present study aimed to evaluate the cytotoxicity of medicinal plant Prosopis cineraria against Brine shrimp. The plant Prosopis cineraria were collected from Acharya Nagarjuna University Campus, Nagarjunanagar, Guntur district, Andhra Pradesh, India. Crude extracts from dried leaves, stem, flowers and roots of P. cineraria were prepared through soxhlet extraction using methanol, chloroform, hexane, ethyl acetate and aqueous sequentially. These extracts were tested in vivo against laboratory cultured brine shrimp larva. The phytochemical screenings were also conducted with standard methods. The methanol, chloroform, hexane, ethyl acetate and aqueous extracts of leaves, stem, flowers and roots shown LC50 values ranging from, 315.00μg/mL to 1425.00μg/mL. Out of the 20 tested extracts, four exhibited moderate toxicity (LC50 100-500μg/mL), 7 extracts displayed weak (LC50 500-1000μg/mL) toxicity, 9 extracts were nontoxic (LC50 >1000μg/mL). The phytochemical screening has revealed the presence of alkaloids, triterpenes, flavonoids, tannins, coumarins, carbohydrates, phenols, saponins, phlobatannins and steroids.

Keywords: Prosopis cineraria, Phyotochemicals, LC50, cytotoxic activity, Brine Shrimp.