DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN CALCIUM AND RAMIPRIL IN TABLET DOSAGE FORMS
Hari Krishna* and Nihar Ranjan Das
ABSTRACT
Objective: A New method was established for simultaneous estimation of Atorvastatin calcium and Ramipril by
RP-HPLC method. Methods: Chromatographic separations were carried using Phenomenex Luna C18 (250 × 4.6
mm, 5μm) column with a mobile phase composition of methanol in addition to phosphate cradle (0.1% v/v
triethylamine pH 4.5 well balanced with 0.1% v/v orthophosphoric harsh) have been delivered at a flow rate of 1
ml/min and the detection was carried out using waters HPLC auto sampler, separation module 2695 HPLC system
with PDA detector at wavelength 254 nm. The running time 12min. Results: The retention time for Atorvastatin
and Ramipril were 3.02 and 6.10 minute respectively. The correlation coefficient values in linearity were found to
be 0.999 and concentration range 20-70 μg/ml for Atorvastatin and 20-70 µg/ml for Ramipril respectively. For
accuracy The total recovery was found to be 99.8 % and 99.8 % for Atorvastatin and Ramipril. LOD and LOQ for
Atorvastatin 2.95 and 9.96. LOD and LOQ for Ramipril 3.34 and 10.05. Conclusion: The results of study showed
that the proposed RP‐HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which
may be useful for the routine estimation of Atorvastatin calcium and Ramipril in tablet dosage form.
Keywords: Atorvastatin calcium, Ramipril, RP?HPLC, Simultaneous estimation.
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