Abstract
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN CALCIUM AND RAMIPRIL IN TABLET DOSAGE FORMS

Hari Krishna* and Nihar Ranjan Das

ABSTRACT

Objective: A New method was established for simultaneous estimation of Atorvastatin calcium and Ramipril by RP-HPLC method. Methods: Chromatographic separations were carried using Phenomenex Luna C18 (250 × 4.6 mm, 5μm) column with a mobile phase composition of methanol in addition to phosphate cradle (0.1% v/v triethylamine pH 4.5 well balanced with 0.1% v/v orthophosphoric harsh) have been delivered at a flow rate of 1 ml/min and the detection was carried out using waters HPLC auto sampler, separation module 2695 HPLC system with PDA detector at wavelength 254 nm. The running time 12min. Results: The retention time for Atorvastatin and Ramipril were 3.02 and 6.10 minute respectively. The correlation coefficient values in linearity were found to be 0.999 and concentration range 20-70 μg/ml for Atorvastatin and 20-70 µg/ml for Ramipril respectively. For accuracy The total recovery was found to be 99.8 % and 99.8 % for Atorvastatin and Ramipril. LOD and LOQ for Atorvastatin 2.95 and 9.96. LOD and LOQ for Ramipril 3.34 and 10.05. Conclusion: The results of study showed that the proposed RP‐HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Atorvastatin calcium and Ramipril in tablet dosage form.

Keywords: Atorvastatin calcium, Ramipril, RP?HPLC, Simultaneous estimation.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • EJBPS: JUNE ISSUE PUBLISHED

    JUNE 2021 Issue has been successfully launched on 1 June 2021

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT