European Journal of Biomedical and Pharmaceutical Sciences

ISOLATION AND IDENTIFICATION OF PATHOGENIC BACTERIA FROM HUSSAIN SAGAR LAKE, HYDERABAD AND THEIR ANTIBIOTIC SENSITIVITY

M. Uma Devi,* A. Bharani, P. Sindhura and S. Thejaswi

ABSTRACT

In the present study water samples were collected from two different sites of Hussain Sagar lake (Hyderabad), and analyzed microbiologically using standard methods. The pathogenic bacteria isolated were identified to be Escherichia coli, Staphylococcus aureus, Bacillus species, Proteus species, Klebsiella pneumoniae, Pseudomonas aeruginosa and actinomycetes member. The presence of pathogenic bacteria can be attributed to discharge of untreated effluents and sewage into the lake. Antibiotic sensitivity test result for four antibiotics ( ampicillin (A) −10 μg, Streptomycin (S) 10 μg, Pencillin (P) 10 μg, and Gentamycin (G) 10 μg) were variable, most species were resistant to Pencillin, Ampicillin and Streptomycin but susceptible to Gentamycin. A better understanding of pathogens that cause infections is important to select appropriate antimicrobials.

Keywords: Pathogenic bacteria, Water sample, Isolation, Antibiotics, Resistance