EVALUATION OF INTERLEUKIN -10 (1082G/A) POLYMORPHISM AMONG SUDANESE PATIENTS WITH SICKLE CELL ANEMIA
Rougia Hamid Alteib Hamid*, Waleed Abdelateif Hussein and Nour Mahmoud Abdelateif Ali
ABSTRACT
Background: Interleukin-10 (IL-10) is a multifunctional cytokine with both immunosuppressive and anti-angiogenic functions and may have both tumor-promoting and -inhibiting properties. We examined the association between a single nucleotide polymorphism (SNP) in IL-10 -1082G/A (rs1800896) among Sudanese sickle cell patients and to assess the association between polymorphisms in IL-10 -1082G/A (rs1800896) and according to age and sex in Sudanese patients with SCA. Methods: A total of 40 patients with sickle cell and 40 control subjects were enrolled in this study. Blood samples were collected from all patients in EDTA containing tubes. Genomic DNA was extracted from all blood samples using salting out method. The genotypic variants of IL-10 (-1082G/A) polymorphism were detected by allele specific-PCR. Results: We found that (70%) of patients have heterogeneous AG genotype, and (17.5%) have homozygous AA genotype and (12.5%) have homozygous GG genotype. GA genotype was significantly associated with higher risk of SCA compared with the homozygous Genotypes (GG and AA), there is no association between IL-10 (-1082G/A) polymorphism and gender and age group. Conclusion: Our study concluded that GA genotype of IL-10 -1082G/A (rs1800896) polymorphism is a risk factor for SCA and G allele is insignificantly higher than a allele in SCA patient.
Keywords: Interleukin-10- polymorphism- Sickle cell anemia
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