European Journal of Biomedical and Pharmaceutical Sciences

CHARACTERISATION OF ANETHOLE COATED IRON OXIDE NANOPARTICLES AND ASSESSMENT OF ITS BIOLOGICAL ACTIVITY

Sampathkumar Banupriya, Krishnamoorthy Kavithaa, Palghat Raghunathan Padma and Sundaravadivelu Sumathi*

ABSTRACT

Synthesis of nanoparticles is carried out with the objective of controlled size, shape and surface coating. There are different forms of nanoparticles such as metallic, non-metallic and oxide. Among these metallic nanoparticles have plenty of applications compared to the bulk materials. The iron oxide nanoparticles were synthesised by co-precipitation method and coated with the compound anethole. Anethole is the compound having anticarcinogenic, antigenotoxic, gastro protective, anti-oxidative, antithrombolytic and vasoactive properties. The coated nanoparticles and uncoated particles were characterised using UV-Visible spectroscopy, Fourier Transform Infrared spectroscopy, X-Ray Diffraction, Energy Dispersive X-ray spectroscopy and Scanning Electron Microscopy analysis to know the absorption peak of the particles, functional group, and nature of the particle and also size of the particles respectively. Further to test its biological activity the antibacterial and antibiofilm activity was assessed using the organisms such as Escherichia coli, Bacillus subtilus, Pseudomonas aeruginosa, Klebsiella pneumonia and Staphylococcus aureus. The coated particles showed more activity compared to the iron oxide nanoparticles. The cytotoxicity of the nanoparticles was assessed using MTT assay and biocompatibility of the particles was assessed using haemolysis assay.

Keywords: Iron oxide nanoparticles, Anethole, Cytotoxicity, Biocompatibility Biofilm, Haemolysis.