POTENTIAL ANTIOXIDANT EFFECT OF A PREPARATION OF TWO PLANTS (DISTEMONANTHUS BENTHAMIANUS AND DISSOTIS ROTUDIFOLIA)
Adélaide Ryta Mande Doue, Cassime Tiemoko*, Bernard Tehi Sea, Honoré N'Guessan Yao, Alain Dit Philippe Bidie
ABSTRACT
Background: Plants are sources of natural antioxidants and some of their compounds have important antioxidant properties and health benefits. Distemonanthus benthamianus and Dissotis rotundifolia are used in traditional medicine for the treatment of many diseases. Objective: To evaluate the in vitro and in vivo antioxidant potential of extracts from a preparation of a mixture of D. benthamianus stem bark and D. rotundifolia leaves in diabetic rats. Methodology: Total phenols and flavonoids in the extracts were determined using spectrophotometric methods. In vitro antioxidant activity was determined by DPPH method and ABTS+ assay. Rats (25) were used for the evaluation of the in vivo antioxidant activity. These animals were divided into 04 batches (05) made diabetic and one control batch (05). After 21 days of treatment with the different extracts, these animals were sacrificed and the markers (SOD, Catalase, MDA and TBars content) were measured. Results: We note a strong presence of phenolic compounds and flavonoids in the hydroethanolic extract compared to the aqueous extract. This extract also showed a strong antioxidant activity in vitro (IC50 = 115.38 ug/ml and % =51.46 mg TE/g) in contrast to the aqueous extract (IC50 = 167.74 ug/ml and % =37.21 mg TE/g). Furthermore, the in vivo activity showed a better regulation of stress markers with the hydroethanolic extract. Conclusion: The extracts seem to have a promising antioxidant potential which can be used as a complementary treatment to various oxidative stress related diseases including diabetes.
Keywords: Antioxidants, plants, phenolic, flavonoids, oxidative damage.
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