Abstract
EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF VARIOUS EXTRACTS OF AERIAL PARTS OF PAVETTA INDICA (LINN)

Abdul Hameed Thayyil, A. Kottai Muthu* and Mohammed Ibrahim

ABSTRACT

The antioxidant activity of various extracts of aerial parts of Pavetta indica (Linn) was investigated in various in-vitro methods. The antioxidant activity was evaluated by Total antioxidant activity (Phosphomolybdic acid method), FRAP assay with reference standard Ascorbate and total flavonoids content respectively. The methanolic extract of Pavetta indica Linnwas found to more effective in the total antioxidant activity. The IC50 values of the methanolic extract of Pavetta indica Linn and ascorbate were found to be 215μg/ml and 410μg/ml respectively. The methanolic extract of Pavetta indica was found more effective in FRAP assay than that of petroleum ether and ethyl acetate extracts. But when compare to the all the three extracts with ascorbate (standard), the methanolic extract of the Pavetta indica showed the better result. The methanolic extract of Pavetta indica contains high amount of flavonoids (3.896 ± 0.014) than that of other two extracts. All the above in vitro studies clearly indicate that the methanolic extract of Pavetta indica has a better antioxidant activity. These in vitro assays indicate that this plant extracts is a better source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.

Keywords: Aerial parts of Pavetta indica, Invitro antioxidant, Total antioxidant activity, FRAP assay, Total flavonoids.


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