European Journal of Biomedical and Pharmaceutical Sciences

ISOLATION OF PHYTOCONSTITUENTS FROM THE ROOTS OF BAMBUSA ARUNDINACEA

H. K. Sundeep Kumar*, M. B. V. Raju, S. Acharyya, B. Mishra

ABSTRACT

The phytochemical examination of the roots of Bambusa arundinacea. The air dried powdered plants materials were separately extracted successively with petroleum ether (600–800C) and ethanol using a soxhlet extractor. The solvents were purified by distillation prior to extraction. The period of extraction was fixed at 48 h for every solvent at every stage of the extraction process. The ethanol extract of Bambusa arundinacea roots upon concentration under reduced pressure left a greasy with brown colour residue (7.2%) which gave positive ferric and Shinoda tests for flavonoids and negative Liebermann-Burchard reaction. TLC examination of the residue over silica gel- G showed number of spots in solvent system: ethyl acetate: ethanol-1: 0.25 on spraying with 5% alcoholic H2SO4 followed by heating. The ethanol residue was subjected to column chromatography over silica gel (120 mesh). Fractions of 200 ml were collected. The residue was subjected to column chromatography over silica gel afforded three compounds named HKS-04, HKS-05 and HKS-06. The compounds were characterized through chemical and spectral analysis and confirmed as 3’, 3, 6, 7-tetramethoxy-4’, 5, 8-trihydroxy flavone (HKS-04), 4-methoxy benzoic acid (HKS-05) and 4’-hydroxy flavan-3-ol (HKS-06) respectively.

Keywords: Bambusa arundinacea, 3’, 3, 6, 7-tetramethoxy-4’, 5, 8-trihydroxy flavone (HKS-04), 4-methoxy benzoic acid (HKS-05) and 4’-hydroxy flavan-3-ol (HKS-06).