PHARMACOLOGICAL SCREENING OF EDIBLE UNRIPE FRUIT EXTRACT OF MUSA BALBISIANA FOR HEPATOPROTECTIVE ACTIVITY
Suresh Arumugam*, Anupam Prahlad, Mohamed Shibli PC, Hashly Parveen, Venkatesh Sellamuthu and Anjitha P.
ABSTRACT
The objective of the present study was to evaluate the hepatoprotective activity of edible unripe fruit extract of Musa balbisiana. The edible unripe fruits were chopped into small pieces and were dried in shade. The powdered drug was extracted with water by cold maceration technique. The extract was subjected to phytochemical analysis. The In-vitro hepatoprotective activity was assessed by performing MTT assay in Hep G2 Cell lines using Paracetamol as the toxicant. Wistar albino rats of female sex and Aqueous extract of Musa balbisiana (AEMB) at 250 mg/kg and 500 mg/kg and Silymarin (100 mg/kg, p. o.) was used for the study. Paracetamol induced hepatotoxicity was employed to evaluate the hepatoprotective potential of the extract. Biochemical parameters and histopathological examination of liver section were performed. Preliminary phytochemical screening showed alkaloids, flavonoids, tannins, phenolic compounds, terpenoids, saponins, presence carbohydrates, proteins, aminoacids, vitamin C, saponin, starch, and quinones. MTT Assay showed that the extract has a significant protective effect on cell lines against hepatotoxicity and it has increased the cell viability in a dose dependent manner which was further confirmed by morphological assessment. AEMB had shown significant hepatoprotective potential by restoring the biochemical parameters. The same was further confirmed using histopathological studies. The results obtained from the study indicate that unripe fruits of Musa balbisiana possess hepatoprotective due to presence of certain constituents like phenols, flavanoids, vitamin C etc.
Keywords: Musa balbisiana, MTT assay, Hepatoprotective.
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