Abstract
METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF CEFTOLOZANE AND TAZOBACTAM BY RP-HPLC METHOD IN PURE AND PHARMACEUTICAL DOSAGE FORM

P. Jyothi Reddy*, Dr. N. Anjaneyulu

ABSTRACT

Objective: A simple, Accurate, precise method was developed for the simultaneous estimation of the Ceftolozane and Tazobactam in pharmaceutical dosage form. Methods: Chromatogram was run through XTerra C18 (4.6 x 150mm, 5 μm particle size). Mobile phase containing Phosphate buffer and Acetonitril in the ratio of 55:45 was pumped through column at a flow rate of 1ml/min. Buffer used at pH 4.6. Temperature was maintained at Ambient. Optimized wavelength for Ceftolozane and Tazobactam was 260 nm. Results: Retention time of Ceftolozane and Tazobactam were found to be 2.28 min and 3.62 min. The % purity of Ceftolozane and Tazobactam was found to be 100.5% and 101.2% respectively. The system suitability parameters for Ceftolozane and Tazobactam such as theoretical plates and tailing factor were found to be 2589.3, 5419.7, 1.11 and 1.34. The resolution was found to be 8.0. The linearity study for Ceftolozane and Tazobactam correlation coefficient (r2) was found to be 0.999 and 0.999, % mean recovery was found to be 100.1% and 100.4%, %RSD for repeatability was 1.2 and 0.60, % RSD for intermediate precision was 1.48 and 0.82 respectively. The precision study was precise, robust and repeatable. LOD value was 0.63 and 0.09, and LOQ value was 1.88 and 0.26respectively. Conclusion: The results of study showed that the proposed RP‐HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Ceftolozane and Tazobactam in pharmaceutical dosage form.

Keywords: Ceftolozane, Tazobactam, RP?HPLC, Simultaneous estimation.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tanima Debnath Sarkar

Download Article : Click here

News & Updation

  • EJBPS: NOVEMBER ISSUE PUBLISHED

    NOVEMBER 2021 Issue has been successfully launched on 1 November 2021

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT