Abstract
CYTOTOXIC RESPONSE OF HCT116 CELL LINE TO MULTIFUNCTIONALIZED MWCNTS IRINOTECAN COMPLEX IN ASSOCIATED WITH MONOCLONAL ANTIBODIES

Hemalatha K. P.*, Suresh V. Kulkarni, Manjunath K. and Ashok Kumar P.

ABSTRACT

Functionalized multi walled carbon nanotube drug delivery system for anticancer drug Irinotecan was designed, Carbon nanotubes functionalized prior to drug and antibodies binding, which makes under biological conditions, multiple functionalization done by binding Bevacizumab antibodies to functionalized multi walled carbon nanotube Irinotecan complex, which promotes through active targeting. Present work is an attempt to investigate the cytotoxic studies of different functionalized MWCNTs by MTT assay. HCT116 were seeded in a 96 well plate, the seeded HCT 116 cells were treated with all the prepared functionalized MWCNTs formulations, MTT assay procedure followed to determine the cell viability of HCT 116 cells. Cells were treated with 10 μL of the formulations, 20 μL of MTT (1 mg/mL) was added to each well and incubated for 3 hours, and then MTT is removed completely from each well, the absorbance was measured at 570 nm to detect the cell viability and cell death. MMS demonstrated an 82.33 % cell death. MWCNTs Covalent functionalized Irinotecan conjugate, MWCNTs Non Covalent functionalized Irinotecan conjugate, MWCNTs Covalent and Non Covalent functionalized Irinotecan conjugate, MWCNTs and both Covalent and Non Covalent functionalized Irinotecan Bevacizumab Antibodies conjugate were cytotoxic with percentage of cell death 52.21 %, 55.15 %, 59.23 % and 73.98 % respectively at 62.5 μg/mL concentration. All the prepared formulations can be used as adjunct therapy for major cancer treatment such as Surgery, radiotherapy and chemotherapy.

Keywords: MWCNTs, MTT Assay, HCT116 Cells, Bevacizumab antibodies, Cytotoxicity, Irinotecan.


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