Abstract
ISOLATION AND CHARACTERIZATION OF BETA –SITOSTEROL FROM THE ETHYL ACETATE EXTRACT FRACTION OF DRIED LEAVES OF SARCOCEPHALUS LATIFOLIUS

*Olaji E.D., Edema M.O., A.P. Oviawe, Iyekowa O., Yakubu A. F. and Olaji C.O.

ABSTRACT

Background: Local fish farmers use the leaves of Sarcocephalus latifolius in rural areas of Southern Nigeria for the treatment of microbial infections of fishes. The plant has diverse folk medicinal uses in traditional medicine, however the main focus of this work is to isolate and spectroscopically characterize the bioactive constituents of the dried leaves of the plant. Sarcocephalus latifolius. Method: The air dried leaves were pulverized to powder and extraction was maintained in soxhlet extractor using methanol solvent. The crude extract was further fractionated using varying solvents of n-hexane, chloroform and ethyl acetate using vacuum liquid chromatography(VLC). The most sensitive fraction (ethyl acetate) based on the antibacterial sensitivity test was subjected to column chromatography and thin layer chromatography. Results: From antibacterial activity, a significant zone of inhibitions at 14mm and 20 mm were recorded against E. coli and P.aeruginosa when compared with the standard drug, tetracycline at 20 mm and 28 mm respectively. A positive test was indicated for the presence of steroid and a white powder compound was isolated which was characterized using combined spectroscopic techniques such as UV, FT-IR, 1HNMR and 13CNMR.The proposed compound was β-sitosterol, a steroid molecule with pharmacological significance in medicine.

Keywords: Sarcocephalus latifolius, steroids, Antimicrobial, ?-sitosterol.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tanima Debnath Sarkar

Download Article : Click here

News & Updation

  • EJBPS: DECEMBER ISSUE PUBLISHED

    DECEMBER 2021 Issue has been successfully launched on 1 December 2021

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT