METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF NETARSUDIL AND LATANOPROST IN BULK AND OPHTHALMIC SOLUTIONS
V. Mounika, N. Susmitha and A. Suneetha*
ABSTRACT
A simple, accurate, precise method was developed for the simultaneous estimation of netarsudil and latanoprost in ophthalmic solutions. Chromatogram was run through Agilent C18 150 x 4.6 mm, 5μ. Mobile phase containing 0.01N potassium dihydrogen ortho phosphate: methanol taken in the ratio 55:45 was pumped through column at a flow rate of 1.0ml/min. Temperature was maintained at 30ºC. Optimized wavelength selected was 220nm. Retention time of netarsudil and latanoprost were found to be 2.589min and 3.221min. %RSD of method precision for netarsudil and latanoprost were found to be 0.7 and 1.4, respectively. % Recovery was obtained 99.86 % and 100.47% for netarsudil and latanoprost, respectively. LOD & LOQ values obtained from regression equations of netarsudil and latanoprost were 0.07, 0.02 and 0.21, 0.07 respectively. Regression equation of netarsudil is y = 11999x + 1319.8 and y = 13329x + 380.64 of latanoprost. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control test in industries.
Keywords: Netarsudil, Latanoprost, RP-HPLC.
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