Abstract
EVALUATION OF ANTIOXIDANT ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACTS OF MYRIANTHUS HOLSTII (CECROPIACEAE) LEAVES, IN VITRO

Yeo Sounta Oumar*, Konan N’dri Seraphin, Silué Kalamourou, Mawa Traoré, Doumbia Idrissa and Coulibaly Adama

ABSTRACT

The purpose of this study is to evaluate the antioxidant activity of Myrianthus holstii (Cecropiaceae), a plant used in traditional medicine against gastroenteritis in western Côte d'Ivoire (Man). The extraction yields of the ethanolic extract (EE) and the aqueous extract are respectively 12.6% and 8.03%. Determination of the total phenol content by the colorimetric method gave the following results: EAfe (50.67 ± 2.08 mg GAE / g of extracts) and EEfe (136.7 ± 2.33 mg GAE / g of extracts). The flavonoid assay using the AlCl3 aluminum chlorides method gave the following results: EAfe (71.33 ± 1.45 mg QE / g extracts) and EEfe (490 ± 17.32 mg QE / g of extracts). The evaluation of the antioxidant activity of extracts was carried out according two methods: the free radical scavenging by the DPPH and the measurement of the reducing power (FRAP). The results obtained indicate that ethanolic extract contains more polyphenolic compounds than the aqueous extract. Ethanolic extract (EAfe) antioxidant properties (EEfe, IC50 = 2.30 ± 0.12 μg / mL) are also greater than those of the aqueous extract (EAfe, IC50 = 4.40 ± 0.60 μg / mL). This antioxidant activity which remains close to that of vitamin C (01.25 ± 0.02) could represent an additional asset in the management of pathologies linked to oxidative stress.

Keywords: Antioxidant Activity, Myrianthus holstii, ethanolic extract leaves (EE).


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