Abstract
AN IMPROVED VALIDATED RP- HPLC METHOD FOR SEPARATION OF CITALOPRAM HBR IMPURITIES IN CITALOPRAM HBR TABLETS

Sandiri Nandeshwari, Dr. Khaja Zeeyauddin and Yagnambhatla Rajendra*

ABSTRACT

Objective: A New method was established for estimation of Citalopram by RP-HPLC method. Methods: Chromatogram was run through Hypersil BDS C18, 250 mm X 4.6 mm, 5 μm column. Mobile phase containing buffer and Methanol in the ratio of 60:40 was pumped through column at a flow rate of 1ml/min. Temperature was maintained at 450C. Optimized wavelength for Citalopram was 239 nm. Results: Retention time of Citalopram was found to be 4.670 min. The % purity of Citalopram was found to be 100.4 %. The system suitability parameters for Citalopram such as theoretical plates and tailing factor were found to be 6.906.6, 1.67. The linearity study for Citalopram was found in concentration range of 20 μg-60 μg correlation coefficient (r2) was found to be 0.999 %, %RSD for repeatability was 0.86, % RSD for intermediate precision was 0.47. The precision study was precise, robust and repeatable. LOD value was 0.598, and LOQ value was 1.81. Conclusion: The results of study showed that the proposed RP‐HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Citalopram in pharmaceutical dosage form.

Keywords: Citalopram, RP?HPLC, Method development, Validation.


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