European Journal of Biomedical and Pharmaceutical Sciences

IDENTIFICATION, ANALYSIS AND CLONING OF SUCROSE SYNTHASE GENE PROMOTER IN PLANT EXPRESSION VECTOR

H. Mubeen*, A. Bashir, S. Farooqi, M. W. Shoaib and S. Raza

ABSTRACT

The study involves identification and analyses of constitutive dicot gene promoter from Solanum lycopersicum isolated through High throughput Genomic Sequences (HTGS). High throughput Genomic Sequences (HTGS) in the GenBank provide one of the information databases for utilizing bioinformatics approaches to identify promoter regions of different genes. The regulatory regions of a particular gene detected on an HTGS can be further screened by different bioinformatics tools to detect cis- regulatory elements, transcription start sites and transcription factor binding sites. A variety of promoters is necessary at all levels of genetic engineering in plants to regulate gene expression. The plant promoters fall into various categories including constitutive, tissue specific, inducible and differentially regulated. The expression of transgenes is regulated by the promoter attached upstream to the gene. The selected SUS promoter sequence was cloned in plant expression vector pGA482. It is proposed that the promoter identified through this study may be utilized to overcome the gene silencing problem by reducing the foreign gene expression to a lower level than the 2X35S.

Keywords: Vector, HTGS, promoter, tissue specific, transcription factor binding sites.