Abstract
EFFECT OF DIRECT ICE-CONTACT INTERFERENCE ON SPERMATIC ACTIVITIES & INTEGRITY IN ALBINO WISTAR RATS.

Olorunfemi Oluwadare Joyce*, Solomon Uvoh Micah, Kiridi Emily Gabriel, Ngaikedi Charles Nnamdi, Inwang Uduak Anthony and Bohr Edward Lete

ABSTRACT

Aim/Background: The primary objective of this research was to evaluate the possible detrimental effect of ice on the epididymis architecture of scrotal testis, and its interactions with sperm parameters. Method: The study was done on adult wistar rats divided into four (4) groups within the weight range of 210-250g.The experimental groups were anaesthetized with 0.5mg/kg of thiopental in normal saline and each rat was administered with the drug, and just after the onset of sleep, the scrotal testis was positioned to lie directly in contact with ice for specific durations. Group I, was the control group that were not anaesthetized while Group II – IV were anaesthetized and ice directly in contact with the scrotal testis for one hour, 45 minutes, and 30 minutes respectively. After this duration of exposure the animals were castrated and semen collected. Spermatozoa were screened for percentage viability, percentage sperm motility, sperm count, percentage dead sperm cells, percentage sluggish sperm cells, and percentage active sperm cells (P < 0.05).Statistical analyses of the data were done using one – way ANOVA and comparisons among treatment groups were made using student t-test. Result: The result of the study indicates that application of ice significantly affected sperm parameters by reducing the percentage motility, sperm count, activeness and sluggishness of sperm cells and also increasing percentage in dead sperm cells and slight increase in percentage sperm viability (P < 0.05). Conclusion: The study suggested, cold and cold shock and their interactions are important sources of variation in sperm parameters and histological changes in the epididymis architecture of the scrotal testis. It is therefore recommended that the environment as it pertains to season should be given urgent attention in human and animal reproduction in order to obtain the best semen quality.

Keywords: ice-contact, epididymis architecture, scrotal testis, spermatozoa, semen.


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